As a Master of Science candidate in Plant Breeding and Biotechnology from the University of Nairobi, I have dedicated my academic journey to unlocking the potential of plant biotechnology which is fueled by my fascination for enhancing agricultural sustainability. My pursuit of excellence began with a Bachelor of Science in Biotechnology and Biosafety from the University of Eldoret. My Master of Science program, supported by the Kenya Climate Smart Agriculture (KCSAP) Project Scholarship, has equipped me with profound insights into innovative plant breeding techniques and biotechnological advancements. My journey is further enriched by a diverse range of experiences. As a Research Assistant at the Kenya Agricultural and Livestock Research Organization's Biotechnology Research Centre, I actively contributed to cutting-edge research projects. This was followed by an impactful stint at the National Biosafety Authority as a Public Service Commission Intern, where I deepened my understanding of regulatory frameworks. My trajectory has now come full circle having returned to the Kenya Agricultural and Livestock Research Organization’s Biotechnology Research Centre, where I continue to explore novel avenues for sustainable agriculture. With a foundation rooted in education, research, and hands-on experience, I am poised to make meaningful contributions to the realm of plant biotechnology and drive positive change in agriculture.
Abstract
Cassava (Manihot esculenta Crantz) stands as a vital carbohydrate source in Africa, yet its potential is hampered by bacterial blight (CBB) and other stresses. To enhance cassava production, understanding the genetic composition and disease resistance of germplasm is crucial. This study, conducted with 15 Kenyan cassava genotypes, employed morphological and molecular markers to evaluate their reaction to CBB. Field trials at Kenyan Agricultural and Livestock Research Organization (KALRO) facilities revealed varied CBB responses, further validated by greenhouse experiments. A total of 30 Start Codon Targeted (SCoT) markers were evaluated for polymorphism, selecting seven for in-depth analysis due to their high polymorphism. Notably, while morphological traits did not distinguish CBB resistance, molecular markers showed a distinct clustering of tolerant varieties. This underscores the role of genetic factors in resistance. These findings play a crucial role in developing and deploying improved cassava genotypes, ultimately benefiting farmers and enhancing food security in Africa and beyond.
Important Links
https://orcid.org/0000-0003-3390-5757?lang=it
Research Supervisors
1. Dr. Juliana J. Cheboi (University of Nairobi) 2. Prof. Douglas Miano (University of Nairobi) 3. Dr. Paul Kuria (Keny
